Laboratorio di Biologia molecolare e Biotecnologie Microbiche

 

Group Leader: Claudio O. Gualerzi

tel.    +39-0737-403240

FAX  +39-0737-403290

e-mail claudio.gualerzi@unicam.it

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Research Line 1.

New antimicrobials targeting translation in bacteria and fungi 

 

 

Publications

The translation initiation functions of IF2: targets for thiostrepton inhibition

L. Brandi, S. Marzi, A. Fabbretti, C. Fleischer, W. Hill, C.O. Gualerzi, S. Lodmell

J. Mol. Biol. 335, 881-894 (2004)     

Novel tetrapeptide inhibitors of bacterial protein synthesis produced by a streptomyces

Brandi, L,  Lazzaroni, A.,. Fabbretti, A.,  Cavalletti, L., Abbondi, M., Corti, E., Ciciliato, I., Gastaldo, L., Marazzi, A., Feroggio, M., Maio, A., Colombo, L. Donadio, S., Marinelli, F., Losi, D., Gualerzi, C.O. and Selva, E. 

Biochemistry 43, 3700-3710 (2006)                   

Specific, efficient and selective inhibition of prokaryotic translation initiation by a novel peptide     antibiotic.

   Brandi, L., Fabbretti, A., La Teana, A., Abbondi, M., Losi, D., Donadio, S. & Gualerzi C.O. , 

   Proc. Nat. Acad. Sci. U.S.A. 103, 39-44 (2006).      

Characterization of GE82832, a peptide translocation inhibitor interacting with bacterial 30S   ribosomal    subunits.

Brandi, L., Fabbretti, A., Di Stefano, M., Lazzaroni A., Abbondi M. and Gualerzi C.O. (2005).

RNA  12, 1262-1270 (2006)      

Methods for identifying compounds that specifically target translation

   Brandi, L., Fabbretti, A., Milon, P., Carotti, M.,  Pon, C.L., & Gualerzi, C.O.

    Meth. Enzymol.  (2007) 431, 229-267                        

Novel assays and novel strains - promising routes to new  antibiotics?

    Donadio, S., Brandi, L., Monciardini, P., Sosio, M. &  Gualerzi C. O. 

          Exp. Opin. Drug Disc.  2, 789-798 (2007).    

Initiation of protein synthesis: a target for antimicrobials.

   Brandi, L., Fabbretti, A., Pon, C.L., Dahlberg, A.E. & Gualerzi, C.O.

   Expert Op. on Therap. Targets (2008) 12, 519-534

 

-   People involved in the research line:

 

Claudio Gualerzi 

Attilio Fabbretti

Letizia Brandi

He Chenguang

      

 

 

Research Line 2.

Translation Initiation in Prokaryotes. 

 

 

Publications

A time-resolved investigation of ribosomal subunit association.

        S.P. Hennelly, A. Antoun, M. Ehrenberg, C.O. Gualerzi, W. Knight, J.S. Lodmell, W.E. Hill J. Mol. Biol.  346, 1243-1258 (2005)   

Solution  structure of the C1-subdomain of Bacillus stearothermophilus translation initiation factor IF2.

H. Wienk, S. Tomaselli, C. Bernard, R. Spurio, D. Picone, C.O. Gualerzi, R. Boelens

Protein Sci. 14,2461-2468 (2005)     

Conformational transitinon of initiation factor 2 from the GTP- to GDP-state visualized on the ribosome.

A:G: Myasnikov, S. Marzi, A. Simonetti, A.M. Giuliodori, C. Gualerzi, G. Yusupova, M. Yusupov, B.P. Klaholz. Nature Struct. Mol. Biol. 12, 1145-1149 (2005)          

Translation initiation factor IF2 interacts with the 30S ribosomal subunit via two separate binding sites

Caserta, E., Tomsic, J., Spurio, R., La Teana, A., Pon, C.L. & Gualerzi, C.O.

J. Mol Biol.  362, 787-799  (2006)        

The nucleotide binding site of bacterial translation initiation factor IF2 as a metabolic sensor

   Milón, P., Tischenko, E., Tomšič, J., Caserta, E., Folkers, G., La Teana, A., Rodnina, M.V., Pon,            C.L., Boelens, R., & Gualerzi, C.O.    Proc. Nat. Acad. Sci. U.S.A. 103, 13962-13967 (2006)           

The real time path of IF3 onto and off the ribosome

  Fabbretti, A. , Pon, C.L., Hennelly, S.P.,. Hill, W.E, Lodmell, J.S. &  Gualerzi, C.O.

 Mol. Cell 25, 285-296 (2007)                                         

Real time dynamics of ribosome-ligands interaction by time-resolved chemical probing methods
          Fabbretti, A., Milon, P., Giuliodori, A.M., Gualerzi C.O. & Pon C.L.

    Meth. Enzymol. (2007) 430, 45-58                             

Transient Kinetics, Fluorescence, and FRET in Studies of Bacterial Initiation

    Milon P., Konevega A., Peske F., Fabbretti., A., Gualerzi, C.O., & Rodnina M.V.

    Meth. Enzymol. (2007) 430,1-30                      

A qualitative kinetic scheme for 70S initiation complex formation

         Grigoriadou, C., Marzi, S. Kirillov, S., Gualerzi, C.O. & Cooperman, B.S.

         J. Mol. Biol. (2007)  373,562-572                               

The translational fidelity function of IF3 during transition from the 30 S Initiation Complex

to the 70 S Initiation Complex. Grigoriadou, C., Marzi, S. Pan, D., Gualerzi, C.O. & Cooperman, B.S.

J. Mol. Biol.  (2007) 373, 551-561                                    

Kinetic checkpoint at a late step in translation initiation

Milon, P., Konevega, A.L. Gualerzi, C.O.& Rodnina M.V.

Mol Cell  (2008) 30, 712-720                        

Structure of a 30S ribosomal initiation complex

             Simonetti, A., Marzi, S., Myasnikov, A.G., Yusupov, M., Gualerzi, C.O. & Klaholz, B.P. Nature (2008)  455, 416-420.

Translation initiation factor IF1 of Bacillus stearothermophilus and Thermus thermophilus substitute or Escherichia coli IF1 in vivo and in vitro without a direct IF1-IF2 interaction

 Kapralou S., Fabbretti A., Garulli  C., Spurio R., Gualerzi C.O., Dahlberg A.E. & Pon C.L.

 Mol. Microbiol. (2008) 70,1368-1377.

Characterization of Bacillus stearothermophilus infA and of its product IF1.

Kapralou S., Fabbretti A., Garulli C., Gualerzi C.O., Pon C.L. & Spurio. R.

Gene (2009) 428, 31-35  

Structural rearrangements in the active site of the Thermus thermophilus 16S rRNA methyltransferase KsgA in a binary complex with 5’-methylthio-adenosine

Demirci, H., Belardinelli, R., Seri, E. Steven T. Gregory, S.T., Gualerzi, C. Dahlberg A.E. & Jogl, G.

J. Mol. Biol. (2009) 388, 271-282

Structural and functional studies of the Thermus thermophilus 16S rRNA Methyltransferase RsmG

Gregory, S.T., Demirci, H., Belardinelli, R., Monshupanee T., Gualerzi  C., Dahlberg A.E, & Jogl, G.

RNA (2009) 15:1693-1704

Sequence of events towards translation initiation complex formation in the crenarchaeon Sulfolobus solfataricus. Hasenoehrl, D., Fabbretti, A., Londei, P., Gualerzi, C.O. & Blaesi, U.

RNA (2009) 15:2288-2298 

Ribosomal interaction of Bacillus stearothermophilus translation initiation factor IF2. Characterization of the active sites. Caserta, E., Ferrara, C., Milon, P., Fabbretti, A., Rocchetti, A., Tomšic, J., Pon, C.L., Gualerzi, C.O. & La Teana, A.    J. Mol. Biol. (2009)

    Ribosome-bound initiation factor 2 recruits initiator tRNA to the 30S initiation complex 

Milon, P., Carotti, M., Konevega, A.L., Wintermeyer, W., Marina V. Rodnina, M.V. & Gualerzi, C.O.

               EMBO Reports (2010)

 

 

 

-   People involved in the research line:

 

Claudio Gualerzi 

Attilio Fabbretti

Riccardo Belardinelli

 

 

 

 

 

 

Research Line 3.

Development of molecular tools for the detection of microbial biodiversity. 

 

 

Meeting report: Molecular ecology workshop. Detection of microbial biodiversity in environmental samples

Metfies, K., Berzano, M., Gualerzi, C., Muyzer, G. and  Medlin, L.

Protist 157, 247-250 (2006)         

 

An optimized protocol for the identification of diatoms, flagellated algae and pathogenic protozoa with phylochips. Metfies, K., Berzano, M., Mayer, C., Roosken, P., Gualerzi, C., Medlin, L. and Muyzer, G.

Molec. Ecol. Notes, 7, 925-936 (2007) 

 

Development of molecular tools for the detection of freshwater diatoms.

   T. N. M. Nguyen, M. Berzano, C. O. Gualerzi, R. Spurio

J. Microb. Meth. 84: 33-40 (2011)

 

Diatom quantification and their distribution with salinity brines in coastal

        sediments of Terra Nova Bay (Antarctica). F. Baldi, C. Facca, D. Marchetto, T.N.M. Nguyen, R. Spurio. Marine Environmental Research. 71: 304-311 (2011)

 

The application of oligonucleotide microarrays and Primer-Probe PCR toward the

identification of freshwater diatoms for water quality assessment.

M. Berzano, S. Marcheggiani, S. Rombini, C.O. Gualerzi, R. Spurio.  Hydrobiologia (2011) accepted for publication.

 

 

 

-   People involved in the research line:

 

Claudio Gualerzi 

Roberto Spurio

Nguyen Thi Nhu Mai

Bidhan Chandra Dhar